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Sartorius VIVAFLOW 200 2,000 MWCO HYDROSART - 248X195X129

Sartorius VIVAFLOW 200 2,000 MWCO HYDROSART - 248X195X129

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  • 1927860
  • VF20H9
Vivaflow® 200 is a ready to use and reusable laboratory crossflow cassette for concentrating up... mehr

Vivaflow® 200 is a ready to use and reusable laboratory crossflow cassette for concentrating up to 5 l samples. The membrane surface per device is 200 cm². One unit comes with all necessary accessories for running the device with a laboratory pump and a size 16 pump head. For speeding up the concentration, two cassettes can be run simultaneously. Vivaflow® 200 is optimally suited for applications like cell culture supernatant concentration, virus concentration and water concentration e.g. in fish breeding facilities. This economical product offers a standard of ease of use, reliability and flexibility unrivalled by any laboratory filtration system in the market. The thin-channel, flip-flow recirculation path of the modules provides high crossflow velocities with minimum pump speed requirements. A single 200 cm2 module typically concentrates 1 l by a factor of 50 in less than 30 min. Alternatively, two Vivaflow® 200 modules can be used in parallel for concentration of 5 l in under 75 minutes. Near total recovery of the concentrate is achieved with a single final rinse. Vivaflow® 200 can be easily cleaned and re-used several times. All accessories for sample concentration, except a peristaltic pump with a pump head for size 16 tubing, are included in the package for using a single cassette. A 500 ml sealed diafiltration reservoir can be added to the system for rapid and convenient buffer exchange. Equipment required The standard Vivaflow® 200 package comes with tubing, pressure indicator, flow restrictor and high pressure pump tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD tubing (size 16). Equipment required The standard Vivaflow® 200 package comes with tubing, pressure indicator, flow restrictor and high pressure pump tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD tubing (size 16). Running 2 Vivaflow® 200 cassettes in parallel For speeding up the concentration, two Vivaflow® 200 cassettes can easily be connected using a Y connector which is preassembeled with the correct tubing sizes and fittings (size 16 tubing for connecting the two Vivaflow® 200 units, size 15 tubing for the pump). Please note that you need a size 15 pump head for running 2 Vivaflows® in parallel. High flow rate and recovery Clear housing for process monitoring Ready to use single cassettes Easy set up of 2 cassettes for use in parallel Re-usable product Performance Characteristics

  Time to concentrate up to 20x (min.) at 3 barinlet pressure, 20°C
 Start volumeAverage fluxRecovery % 
 1 litreml/minDirect25 ml rinse
Lysozyme 0.25 mg/ml (14,000 MW)
2,000 MWCO Hydrosart160697 %>99 %
3,000 MWCO PES180597 %>99 %
BSA 1.0 mg/ml (66,000 MW)
5,000 MWCO PES293398 %>99 %
5,000 MWCO Hydrosart701498 %>99 %
10,000 MWCO PES234196 %>99 %
10,000 MWCO Hydrosart352798 %>99 %
30,000 MWCO PES253896 %99 %
30,000 MWCO Hydrosart204896 %>99 %
50,000 MWCO PES224396 %98 %
γ Globulins 1.0 mg/ml (average 160,000 MW)
100,000 MWCO PES541896 %99 %
100,000 MWCO RC452196 %99 %
Yeast 1.0 mg/ml (S. Cerevisiae)
0.2 μm PES118692 %98 %
Dilute solute concentration, start volume 1 litre at 3 bar, 10,000 MWCO PES
BSA 0.001 mg/ml185290 %98 %
BSA 0.01 mg/ml204792 %98 %
BSA 0.1 mg/ml214594 %99 %
Start volume 5 litres (two VF200 in parallel at 3 bar) 10,000 MWCO PES
BSA 1.0 mg/ml (66,000 MW)677097 %>99 %

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Sartorius

Die Kunden von Sartorius arbeiten hart daran, Medikamente zu entwickeln und herzustellen, die das Fortschreiten von Krankheiten heilen, verhindern oder stoppen. Um erfolgreich zu sein, verlassen sie sich auf Partner, die ihre aktuellen und zukünftigen Herausforderungen verstehen. Seit 150 Jahren steht die Marke Sartorius für innovative Produkte und Lösungen, die die Arbeit von Wissenschaftlern und Ingenieuren weltweit unterstützen. Dabei hat Satorius sich zunehmend darauf konzentriert, Durchbrüche in der Life-Science-Forschung und der Bioproduktion zu ermöglichen.

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