Sartorius VIVAFLOW 200 SYSTEM(115v) EASY LOAD - 240X180X180
- Bestell-Nr.: 1927869
- Hersteller-Art.-Nr.: VFS204
Vivaflow® 200 is a ready to use and reusable laboratory crossflow cassette for concentrating up to 5 l samples. The membrane surface per device is 200 cm². One unit comes with all necessary accessories for running the device with a laboratory pump and a size 16 pump head. For speeding up the concentration, two cassettes can be run simultaneously. Vivaflow® 200 is optimally suited for applications like cell culture supernatant concentration, virus concentration and water concentration e.g. in fish breeding facilities. This economical product offers a standard of ease of use, reliability and flexibility unrivalled by any laboratory filtration system in the market. The thin-channel, flip-flow recirculation path of the modules provides high crossflow velocities with minimum pump speed requirements. A single 200 cm2 module typically concentrates 1 l by a factor of 50 in less than 30 min. Alternatively, two Vivaflow® 200 modules can be used in parallel for concentration of 5 l in under 75 minutes. Near total recovery of the concentrate is achieved with a single final rinse. Vivaflow® 200 can be easily cleaned and re-used several times. All accessories for sample concentration, except a peristaltic pump with a pump head for size 16 tubing, are included in the package for using a single cassette. A 500 ml sealed diafiltration reservoir can be added to the system for rapid and convenient buffer exchange. Equipment required The standard Vivaflow® 200 package comes with tubing, pressure indicator, flow restrictor and high pressure pump tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD tubing (size 16). Equipment required The standard Vivaflow® 200 package comes with tubing, pressure indicator, flow restrictor and high pressure pump tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD tubing (size 16). Running 2 Vivaflow® 200 cassettes in parallel For speeding up the concentration, two Vivaflow® 200 cassettes can easily be connected using a Y connector which is preassembeled with the correct tubing sizes and fittings (size 16 tubing for connecting the two Vivaflow® 200 units, size 15 tubing for the pump). Please note that you need a size 15 pump head for running 2 Vivaflows® in parallel. High flow rate and recovery Clear housing for process monitoring Ready to use single cassettes Easy set up of 2 cassettes for use in parallel Re-usable product Performance Characteristics
| Time to concentrate up to 20x (min.) at 3 bar inlet pressure, 20°C | ||||
|---|---|---|---|---|
| Start volume | Average flux | Recovery % | ||
| 1 litre | ml/min | Direct | 25 ml rinse | |
| Lysozyme 0.25 mg/ml (14,000 MW) | ||||
| 2,000 MWCO Hydrosart | 160 | 6 | 97 % | > 99 % |
| 3,000 MWCO PES | 180 | 5 | 97 % | > 99 % |
| BSA 1.0 mg/ml (66,000 MW) | ||||
| 5,000 MWCO PES | 29 | 33 | 98 % | > 99 % |
| 5,000 MWCO Hydrosart | 70 | 14 | 98 % | > 99 % |
| 10,000 MWCO PES | 23 | 41 | 96 % | > 99 % |
| 10,000 MWCO Hydrosart | 35 | 27 | 98 % | > 99 % |
| 30,000 MWCO PES | 25 | 38 | 96 % | 99 % |
| 30,000 MWCO Hydrosart | 20 | 48 | 96 % | > 99 % |
| 50,000 MWCO PES | 22 | 43 | 96 % | 98 % |
| γ Globulins 1.0 mg/ml (average 160,000 MW) | ||||
| 100,000 MWCO PES | 54 | 18 | 96 % | 99 % |
| 100,000 MWCO RC | 45 | 21 | 96 % | 99 % |
| Yeast 1.0 mg/ml (S. Cerevisiae) | ||||
| 0.2 μm PES | 11 | 86 | 92 % | 98 % |
| Dilute solute concentration, start volume 1 litre at 3 bar, 10,000 MWCO PES | ||||
| BSA 0.001 mg/ml | 18 | 52 | 90 % | 98 % |
| BSA 0.01 mg/ml | 20 | 47 | 92 % | 98 % |
| BSA 0.1 mg/ml | 21 | 45 | 94 % | 99 % |
| Start volume 5 litres (two VF200 in parallel at 3 bar) 10,000 MWCO PES | ||||
| BSA 1.0 mg/ml (66,000 MW) | 67 | 70 | 97 % | > 99 % |
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