Sartorius Vivacon 500-PCR Grade 30,000 MWC 500/BOX - 0X0X0
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- Order No.: 1919106
- Manufacturer Art. No.: VN01H23ETO
Vivacon 500 is optimized for the concentration / recovery and buffer exchange applications of genomic DNA and peptides for samples up to 500 µl initial volume. They are also the product of choice for peptide fractionation applications in this volume range. For optimal performance with highly diluted samples, e.g. forensic specimens, Vivacon 500 is equipped with patented Hydrosart® regenerated cellulose membranes with extremely low unspecific binding properties and high flux. Selected MWCOs are available sterile and DNA free (PCR Grade units). They have been treated with a dual-cycle ethylene oxide (ETO) gas process to ensure they are sterile to a safety assurance level of 106 and free of detectable human genomic DNA contamination. Tests were performed with a qPCR assay over 55 cycles with a verified detection limit of 2 pg.High recoveries and excellent reproducibility are paired with convenience offered by volume graduation and molecular weight cut-offs printed on individual devices. Vivacon 500 features a horizontal membrane design optimal for DNA fractionation (primer removal) as well as peptide fractionation (Filter Aides Sample Preparation, FASP1). The possibility of a reverse spin after sample processing assures complete concentrate recovery which is especially important when working with low sample concentrations.1Wisniewski JR, Zougman A, Nagaraj N, Mann M. Universal sample preparation method for proteome analysis (2009). Nat Methods. 6(5):359-62. Concentration of up to 0.5 ml genomic DNA or peptide samples Low binding material Reverse spin possibility MWCO printed on Complete and highly reproducible concentrate recovery Selected cut offs available sterile and DNA free* for e.g. forensic applications * qPCR assay over 55 cycles with a verified limit of detection < 2 pg Performance Characteristics for DNA Start volume 0.5 ml, sample concentration 50 ng/ml.
| Sample size (bp) | Time to concentrate up to 30x [min.] at 20°C | Concentrate recovery % | g-force (xg) | |
|---|---|---|---|---|
| 2,000 MWCO | 10 | 60 min. | 93 % | 7,500 |
| 10,000 MWCO | 30 | 25 min. | 94 % | 7,500 |
| 30,000 MWCO | 50 | 18 min. | 88 % | 5,000 |
| 50,000 MWCO | 300 | 18 min. | 91 % | 5,000 |
| 100,000 MWCO | 600 | 10 min. | 87 % | 3,000 |
| 125,000 MWCO | 650 | 12 min. | 85 % | 2,000 |
| 125,000 MWCO | 900 | 9 min. | 94 % | 3,000 |
| Sample | Time to concentrate upto 30x [min.] at 20°C | Concentraterecovery % | g-force (xg) | |
|---|---|---|---|---|
| 2,000 MWCO | 0.25 mg/mlcytochrome c | 30 min. | 95 % | 14,000 |
| 10,000 MWCO | 0.25 mg/mlcytochrome c | 15 min. | 92 % | 14,000 |
| 30,000 MWCO | 1.0 mg/ml BSA | 10 min. | 95 % | 14,000 |
| 50,000 MWCO | 1.0 mg/ml BSA | 10 min. | 92 % | 14,000 |
| 100,000 MWCO | 1.0 mg/mlbovine IgG | 11 min. | 90 % | 8,000 |
| 125,000 MWCO | 1.0 mg/mlbovine IgG | 10 min. | 81 % | 8,000 |
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