Sartorius Vivaflow 200 Hydrosart, 100 kDa - 248X195X129
- Order No.: 2504419
- Manufacturer Art. No.: VF20H4
Vivaflow® 200 is a ready to use and reusable laboratory crossflow cassette for concentrating up to 5 l samples. The membrane surface per device is 200 cm². One unit comes with all necessary accessories for running the device with a laboratory pump and a size 16 pump head. For speeding up the concentration, two cassettes can be run simultaneously. Vivaflow® 200 is optimally suited for applications like cell culture supernatant concentration, virus concentration and water concentration e.g. in fish breeding facilities. This economical product offers a standard of ease of use, reliability and flexibility unrivalled by any laboratory filtration system in the market. The thin-channel, flip-flow recirculation path of the modules provides high crossflow velocities with minimum pump speed requirements. A single 200 cm2 module typically concentrates 1 l by a factor of 50 in less than 30 min. Alternatively, two Vivaflow® 200 modules can be used in parallel for concentration of 5 l in under 75 minutes. Near total recovery of the concentrate is achieved with a single final rinse. Vivaflow® 200 can be easily cleaned and re-used several times. All accessories for sample concentration, except a peristaltic pump with a pump head for size 16 tubing, are included in the package for using a single cassette. A 500 ml sealed diafiltration reservoir can be added to the system for rapid and convenient buffer exchange. Equipment required The standard Vivaflow® 200 package comes with tubing, pressure indicator, flow restrictor and high pressure pump tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD tubing (size 16). Equipment required The standard Vivaflow® 200 package comes with tubing, pressure indicator, flow restrictor and high pressure pump tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD tubing (size 16). Running 2 Vivaflow® 200 cassettes in parallel For speeding up the concentration, two Vivaflow® 200 cassettes can easily be connected using a Y connector which is preassembeled with the correct tubing sizes and fittings (size 16 tubing for connecting the two Vivaflow® 200 units, size 15 tubing for the pump). Please note that you need a size 15 pump head for running 2 Vivaflows® in parallel. High flow rate and recovery Clear housing for process monitoring Ready to use single cassettes Easy set up of 2 cassettes for use in parallel Re-usable product Performance Characteristics
| Time to concentrate up to 20x (min.) at 3 barinlet pressure, 20°C | ||||
|---|---|---|---|---|
| Start volume | Average flux | Recovery % | ||
| 1 litre | ml/min | Direct | 25 ml rinse | |
| Lysozyme 0.25 mg/ml (14,000 MW) | ||||
| 2,000 MWCO Hydrosart | 160 | 6 | 97 % | >99 % |
| 3,000 MWCO PES | 180 | 5 | 97 % | >99 % |
| BSA 1.0 mg/ml (66,000 MW) | ||||
| 5,000 MWCO PES | 29 | 33 | 98 % | >99 % |
| 5,000 MWCO Hydrosart | 70 | 14 | 98 % | >99 % |
| 10,000 MWCO PES | 23 | 41 | 96 % | >99 % |
| 10,000 MWCO Hydrosart | 35 | 27 | 98 % | >99 % |
| 30,000 MWCO PES | 25 | 38 | 96 % | 99 % |
| 30,000 MWCO Hydrosart | 20 | 48 | 96 % | >99 % |
| 50,000 MWCO PES | 22 | 43 | 96 % | 98 % |
| γ Globulins 1.0 mg/ml (average 160,000 MW) | ||||
| 100,000 MWCO PES | 54 | 18 | 96 % | 99 % |
| 100,000 MWCO RC | 45 | 21 | 96 % | 99 % |
| Yeast 1.0 mg/ml (S. Cerevisiae) | ||||
| 0.2 μm PES | 11 | 86 | 92 % | 98 % |
| Dilute solute concentration, start volume 1 litre at 3 bar, 10,000 MWCO PES | ||||
| BSA 0.001 mg/ml | 18 | 52 | 90 % | 98 % |
| BSA 0.01 mg/ml | 20 | 47 | 92 % | 98 % |
| BSA 0.1 mg/ml | 21 | 45 | 94 % | 99 % |
| Start volume 5 litres (two VF200 in parallel at 3 bar) 10,000 MWCO PES | ||||
| BSA 1.0 mg/ml (66,000 MW) | 67 | 70 | 97 % | >99 % |
Sartorius customers are working hard to develop and manufacture medicines that cure, prevent or stop the progression of diseases. To be successful, they rely on partners who understand their current and future challenges. For 150 years, the Sartorius brand has stood for innovative products and solutions that support the work of scientists and engineers worldwide. Satorius has increasingly focused on enabling breakthroughs in life science research and bioproduction.